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th17 cell polarization medium  (R&D Systems)


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    R&D Systems th17 cell polarization medium
    Th17 Cell Polarization Medium, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/flowx+human+th17+cell+multi+color+flow+cytometry+kit/FlowX+Human+Th17+Cell+Multi-Color+Flow+Cytometry+Kit/pm40089498-394-0-9
    Average 93 stars, based on 11 article reviews
    th17 cell polarization medium - by Bioz Stars, 2026-10
    93/100 stars

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    Flow Cytometry:

    Article Title: Longitudinal variation of serum PCSK9 in ulcerative colitis: association with disease activity, T helper 1/2/17 cells, and clinical response of tumor necrosis factor inhibitor.
    Article Snippet: Aims Proprotein convertase subtilisin/kexin type 9 (PCSK9) modulates CD4+ T cell differentiation and inflammatory response, the latter ones mediate ulcerative colitis (UC) initiation.. This study intended to explore the correlation of serum PCSK9 with disease activity, T helper (h)1/Th2/Th17 cells, and clinical response of tumor necrosis factor inhibitor (TNFi) in UC patients.. Methods In 65 UC patients underwent TNFi treatment, serum PCSK9 was evaluated at baseline (W0), week (W)2, W6, and W12 by enzyme-linked immunosorbent assays; meanwhile, Th1/Th2/Th17 cells were determined at W0 by flow cytometry.

    Article Title: Serum retinol-binding protein 4 in stroke patients: correlation with T helper 17/regulatory T cell imbalance and 3-year cognitive function decline
    Article Snippet: First, CD4 + T cells were separated from the PB samples using a DynabeadsTM FlowCompTM Human CD4 kit (No. Cat. 11361D, Thermo Fisher Scientific, Inc., Waltham, Massachusetts, United States). .. Then, the proportions of Th17 and Treg cells in CD4 + T cells were determined by flow cytometry (FCM) using the FlowX Human Th17 Cell Multi-Color Flow Cytometry Kit (No. Cat. FMC007B, R&D Systems, Inc., Minneapolis, Minnesota, United States) and the Regulatory T Cell (Treg) Flow Cytometry Panel (No. Cat. FMC-P-004, R&D Systems, Inc., Minneapolis, Minnesota, USA). ..



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    R&D Systems th17 cell polarization medium
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    https://www.bioz.com/product/flowx+human+th17+cell+multi+color+flow+cytometry+kit/FlowX+Human+Th17+Cell+Multi-Color+Flow+Cytometry+Kit/pm40089498-394-0-9
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    a. Schematic of the <t>Th17</t> and iTreg differentiation protocol for primary human naïve CD4 + CD25 - T cells isolated from the umbilical cord blood of healthy neonates. CD4 + CD25 - T cells were activated with anti-CD3/anti-CD28 and differentiated into Th17 or iTreg cells in the presence of corresponding cytokines for 3 days. DMSO control or conjugated bile acids (Asn-UDCA, Ser-CDCA, Tyr-CDCA, and unconjugated UDCA) at 100 µM were added on day 0 of differentiation. b-e. IL-17a secretion in the supernatant of Th17 cultures treated with Asn-UDCA ( b ), Ser-CDCA ( c ), Tyr-CDCA ( d ), and UDCA ( e ) was quantified on day 3 of differentiation from four biological replicates using ELISA. f-i. Intracellular Foxp3 protein expression in iTregs cultured with Asn-UDCA ( f ), Ser-CDCA ( g ), Tyr-CDCA ( h ), or UDCA ( i ) was assessed on day 3 of differentiation by flow cytometry. Geometric mean fluorescence intensity (MFI) values are shown for four biological replicates. Statistical significance was determined using paired, two-tailed Student’s t-test.
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    a. Schematic of the <t>Th17</t> and iTreg differentiation protocol for primary human naïve CD4 + CD25 - T cells isolated from the umbilical cord blood of healthy neonates. CD4 + CD25 - T cells were activated with anti-CD3/anti-CD28 and differentiated into Th17 or iTreg cells in the presence of corresponding cytokines for 3 days. DMSO control or conjugated bile acids (Asn-UDCA, Ser-CDCA, Tyr-CDCA, and unconjugated UDCA) at 100 µM were added on day 0 of differentiation. b-e. IL-17a secretion in the supernatant of Th17 cultures treated with Asn-UDCA ( b ), Ser-CDCA ( c ), Tyr-CDCA ( d ), and UDCA ( e ) was quantified on day 3 of differentiation from four biological replicates using ELISA. f-i. Intracellular Foxp3 protein expression in iTregs cultured with Asn-UDCA ( f ), Ser-CDCA ( g ), Tyr-CDCA ( h ), or UDCA ( i ) was assessed on day 3 of differentiation by flow cytometry. Geometric mean fluorescence intensity (MFI) values are shown for four biological replicates. Statistical significance was determined using paired, two-tailed Student’s t-test.
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    a. Schematic of the <t>Th17</t> and iTreg differentiation protocol for primary human naïve CD4 + CD25 - T cells isolated from the umbilical cord blood of healthy neonates. CD4 + CD25 - T cells were activated with anti-CD3/anti-CD28 and differentiated into Th17 or iTreg cells in the presence of corresponding cytokines for 3 days. DMSO control or conjugated bile acids (Asn-UDCA, Ser-CDCA, Tyr-CDCA, and unconjugated UDCA) at 100 µM were added on day 0 of differentiation. b-e. IL-17a secretion in the supernatant of Th17 cultures treated with Asn-UDCA ( b ), Ser-CDCA ( c ), Tyr-CDCA ( d ), and UDCA ( e ) was quantified on day 3 of differentiation from four biological replicates using ELISA. f-i. Intracellular Foxp3 protein expression in iTregs cultured with Asn-UDCA ( f ), Ser-CDCA ( g ), Tyr-CDCA ( h ), or UDCA ( i ) was assessed on day 3 of differentiation by flow cytometry. Geometric mean fluorescence intensity (MFI) values are shown for four biological replicates. Statistical significance was determined using paired, two-tailed Student’s t-test.
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    Image Search Results


    a. Schematic of the Th17 and iTreg differentiation protocol for primary human naïve CD4 + CD25 - T cells isolated from the umbilical cord blood of healthy neonates. CD4 + CD25 - T cells were activated with anti-CD3/anti-CD28 and differentiated into Th17 or iTreg cells in the presence of corresponding cytokines for 3 days. DMSO control or conjugated bile acids (Asn-UDCA, Ser-CDCA, Tyr-CDCA, and unconjugated UDCA) at 100 µM were added on day 0 of differentiation. b-e. IL-17a secretion in the supernatant of Th17 cultures treated with Asn-UDCA ( b ), Ser-CDCA ( c ), Tyr-CDCA ( d ), and UDCA ( e ) was quantified on day 3 of differentiation from four biological replicates using ELISA. f-i. Intracellular Foxp3 protein expression in iTregs cultured with Asn-UDCA ( f ), Ser-CDCA ( g ), Tyr-CDCA ( h ), or UDCA ( i ) was assessed on day 3 of differentiation by flow cytometry. Geometric mean fluorescence intensity (MFI) values are shown for four biological replicates. Statistical significance was determined using paired, two-tailed Student’s t-test.

    Journal: medRxiv

    Article Title: Trajectories of microbiome-derived bile acids in early life – insights into the progression to islet autoimmunity

    doi: 10.1101/2025.02.18.25322275

    Figure Lengend Snippet: a. Schematic of the Th17 and iTreg differentiation protocol for primary human naïve CD4 + CD25 - T cells isolated from the umbilical cord blood of healthy neonates. CD4 + CD25 - T cells were activated with anti-CD3/anti-CD28 and differentiated into Th17 or iTreg cells in the presence of corresponding cytokines for 3 days. DMSO control or conjugated bile acids (Asn-UDCA, Ser-CDCA, Tyr-CDCA, and unconjugated UDCA) at 100 µM were added on day 0 of differentiation. b-e. IL-17a secretion in the supernatant of Th17 cultures treated with Asn-UDCA ( b ), Ser-CDCA ( c ), Tyr-CDCA ( d ), and UDCA ( e ) was quantified on day 3 of differentiation from four biological replicates using ELISA. f-i. Intracellular Foxp3 protein expression in iTregs cultured with Asn-UDCA ( f ), Ser-CDCA ( g ), Tyr-CDCA ( h ), or UDCA ( i ) was assessed on day 3 of differentiation by flow cytometry. Geometric mean fluorescence intensity (MFI) values are shown for four biological replicates. Statistical significance was determined using paired, two-tailed Student’s t-test.

    Article Snippet: After differentiation, secreted IL-17a levels were determined from Th17 cell-culture supernatants at 72 hours using the human IL-17a DuoSet ELISA kit (R&D Systems, Cat# DY317-05, DY008).

    Techniques: Isolation, Control, Enzyme-linked Immunosorbent Assay, Expressing, Cell Culture, Flow Cytometry, Fluorescence, Two Tailed Test